Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link from Abcam

Product Specs
ItemBiotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab201795
Quantity1 MG, 3 X10UG, 3 X100UG, 100 UG
ApplicationsConjugation
TargetBiotin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Serological Investigation of Persistent Villous Atrophy in Celiac DiseaseClinical and Translational GastroenterologyDecember 1, 2023Changlin Gong, Claudia Saborit, Xin Long, Ao Wang, Beishi Zheng, Howard Chung, Suzanne K. Lewis, Suneeta Krishnareddy, Govind Bhagat, Peter H.R. Green, et al.as the capture antibody and ab242896 as the detector antibody (Abcam). A biotinylation kit (Abcam, ab201795) was used to label the detector antibody with biotin. Recombinant human apolipoprotein A-IV/apo A4 protein

(2) Short-Stranded Zein Fibers for Muscle Tissue Engineering in Alginate-Based Composite HydrogelsGelsNovember 17, 2023Lea Melzener, Sergio Spaans, Nicolas Hauck, André J. G. Pötgens, Joshua E. Flack, Mark J. Post, Arın Doğancarrier and preservation-free antibody was performed with the Abcam Lightning Link biotinylation kit, ab201795), and the respective secondary antibody was added (Supplementary Table S2). After a final wash, signals

(3) Extracellular Hsp90 Binds to and Aligns Collagen-1 to Enhance Breast Cancer Cell InvasivenessCancers (Basel)October 31, 2023Pragya Singh, Varshini Ramanathan, Yang Zhang, Irene Georgakoudi, Daniel G. JayCollagen-1 binding assay, 100 μg of protein was biotinylated using an Abcam biotinylation kit (Abcam Ab201795, Cambridge, UK) following the manufacturer’s protocol. Once biotinylated, excess unbound biotin was

(4) Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3Fluids and Barriers of the CNSAugust 15, 2023Niklas Ortenlöf, Suvi Vallius, Helena Karlsson, Claes Ekström, Amanda Kristiansson, Bo Holmqvist, Olga Göransson, Magdaléna Vaváková, Martin Rydén, Galen Carey, et al.Alexa Fluor® 647 Conjugation Kit (Fast, Lightning-Link®, ab269823, Abcam, Cambridge, UK), Biotin Conjugation Kit (Fast, Type A, Lightning-Link, ab201795, Abcam) and FITC Conjugation Kit (Fast, Lightning-Link, ab188285, Abcam) according to the manufacturer’s

(5) TRAPnSeq allows high-throughput profiling of antigen-specific antibody-secreting cellsCell Reports MethodsJuly 7, 2023Seblewongel Asrat, Joseph C. Devlin, Andrea Vecchione, Brian Klotz, Ian Setliff, Devin Srivastava, Andre Limnander, Ashique Rafique, Christina Adler, Stephen Porter, et al.and streptavidin conjugation of capture reagents Biotin Conjugation Kit, Lightning-Link (Abcam, cat: ab201795) was used to conjugate antigens to biotin following manufacturer’s instructions. α-Igk and FceRIα ectodomain

(6) Immunothrombosis biomarkers as potential predictive factors of acute respiratory distress syndrome in moderate-to-critical COVID-19: A single-center, retrospective cohort studyImmunology LettersFebruary 1, 2023Giou-Teng Yiang, Yao-Kuang Wu, Kuo-Wang Tsai, I-Shiang Tzeng, Wan-Chung Hu, Min-Tser Liao, Kuo-Cheng Lu, Hsueh-Wen Chung, You-Chen Chao, Wen-Lin Suantibodies had not been conjugated with biotin. Therefore, a biotinylation kit/biotin conjugation kit (ab201795, Abcam, UK) was used to aid conjugation. This was followed by the addition of streptavidin HRP solution,

(7) Enzyme linked oligonucleotide assay for the sensitive detection of SARS-CoV-2 variantsFrontiers in Cellular and Infection MicrobiologySeptember 29, 2022Michael Shola David, Damira Kanayevabiotinylated using a biotinylation kit/biotin conjugation kit (fast, type A) – lightning-link® (Abcam, ab201795) according to the manufacturer’s protocol. In brief, 1 μL of a modifier reagent in the kit was added

(8) Quantitative and ultrasensitive in situ immunoassay technology for SARS-CoV-2 detection in salivaScience advancesMay 27, 2022Chen Y, Liu F, Lee LP.virus H1N1 hemagglutinin (HA) antibody (GTX127357, GeneTex) were biotinylated via a biotinylation kit (ab201795, Abcam) as per the manufacturer’s protocol. The S1 and S2 antibodies were labeled with streptavidin-conjugated

(9) Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8mBioApril 26, 2022Wu X, Xia T, Shin WJ, Yu KM, Jung W, Herrmann A, Foo SS, Chen W, Zhang P, Lee JS, et al.and biotinylated using the Biotinylation kit/Biotin Conjugation kit (Fast, Type A)-Lightning-Link (ab201795; Abcam) and used as the detection antibody. A 96-well ELISA plate was coated with 1 μg/mL monoclonal

(10) Fortilin interacts with TGF-β1 and prevents TGF-β receptor activationCommunications biologyFebruary 23, 2022Pinkaew D, Martinez-Hackert E, Jia W, King MD, Miao F, Enger NR, Silakit R, Ramana K, Chen SY, Fujise K.fortilin, we first biotinylated α-fortilin mAb using the Lighting-Link® Biotinylation Kit (Catalog #: ab201795, Abcam). We then coated the wells of a 96-well plate (Clear Flat Bottom Polystyrene High Bind Microplate,

(11) Sublytic C5b-9 Induces CCL3/4 Production and Macrophage Accumulation in Thy-1N Rats via PKC-α/p65/IRF-8 AxisInternational journal of biological sciencesJanuary 1, 2022Wang W, Qian B, Zhao C, Peng M, Liu L, Xie M, Peng N, He Q, Ying S, Zhu Y, et al.from R&D Systems (USA) and Thermo Fisher Scientific (USA), respectively. A biotin conjugation kit (ab201795) and Streptavidin-HRP (ab7403) was supplied by Abcam. An EZ-ChIPTM chromatin immunoprecipitation kit

(12) Ultrasensitive, high-throughput, and rapid simultaneous detection of SARS-CoV-2 antigens and IgG/IgM antibodies within 10 min through an immunoassay biochipMicrochimica ActaAugust 1, 2021Chunhua Wang, Chao Wang, Jiaoyan Qiu, Jianwei Gao, Hong Liu, Yu Zhang, Lin Hanpurification. The detection antibodies of S-antigen and N-antigen were labeled with biotin using commercial kits (ab201795-Biotin, Abcam). Briefly, 100 μL of Biotin Modifier regent was added into lyophilized detection antibodies

(13) Active Ratio Test (ART) as a Novel Diagnostic for Ovarian CancerDiagnostics (Basel)June 7, 2021Sung-Woog Kang, Adam Rainczuk, Martin K. Oehler, Thomas W. Jobling, Magdalena Plebanski, Andrew N. StephensMonoclonal Antibodies mAb-RA2 and mAb-RG2 antibodies were biotinylated using a biotinylation kit (Abcam; ab201795) according to the manufacturer’s instructions. In brief, 10 µL of biotin modifier reagent was added

(14) Validating METCAM/MUC18 as a Novel Biomarker to Predict the Malignant Potential of Prostate Cancer at an Early Stage by Using a Modified Gold Nanoparticles-Based Lateral Flow ImmunoassayDiagnostics (Basel)March 4, 2021Jui-Chuang Wu, Yin-Huan Chuang, Yu-Chun Wei, Chia-Chi Hsieh, Yuan-Hung Pong, Yenn-Rong Su, Vincent F.-S. Tsai, Guang-Jer Wuchicken antibody was biotinylated by the using the biotin (Type A) Fast Conjugation Kit from Abcam (ab201795-300ug) [21–23]. The epitope-recognition of the antibody was not affected [Manual for antibody conjugation

(15) Quantitative and Ultrasensitive In-situ Immunoassay Technology for SARS-CoV-2 Detection in SalivaResearch squareJanuary 18, 2021Lee L, Liu F, Chen Y, Roma G.Influenza A virus H1N1 HA (Hemagglutinin) antibody (GTX127357, GeneTex) were biotinylated via a biotinylation kit (ab201795, Abcam) as per manufacturer protocol. The S1 and S2 antibodies were labeled with streptavidin conjugated

(16) Small Molecules Target the Interaction between Tissue Transglutaminase and FibronectinMolecular cancer therapeuticsJune 1, 2019Sima LE, Yakubov B, Zhang S, Condello S, Grigorescu AA, Nwani NG, Chen L, Schiltz GE, Arvanitis C, Zhang ZY, et al.binding region (Sigma, #F0162) was biotinylated using Biotin (Type A) Fast Conjugation Kit (Abcam, #ab201795). Biotinylated FN45 (10 μg/mL in PBS) was captured on pre-hydrated streptavidin-coated sensors (Pall

(17) Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier IntegrityImmunityFebruary 19, 2019Martínez-López M, Iborra S, Conde-Garrosa R, Mastrangelo A, Danne C, Mann ER, Reid DM, Gaboriau-Routhiau V, Chaparro M, Lorenzo MP, et al.Commercial Assays,Critical Commercial Assays,Critical Commercial Assays ,, Biotinylation Kit,Abcam,Cat# ab201795 Bacteria Counting Kit,ThermoFisher,Cat# B7277 RNeasy Mini Kit,QIAGEN,Cat# 74104 High-Capacity cDNA

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12/17/2021Reliable Conjugation Kit Karl WagnerIt takes less than half an hour of incubation time.

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link from Abcam

Abcam

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Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link from Abcam

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